ĪLTEX - Alternatives to animal experimentation: Vol. 1 (2010)Ĭopenhagen: Improving in vitro methods by developing and using defined culture mediaĪdvanced Good Cell Culture Practice for human primary, stem cell-derived and organoid models as well as microphysiological systemsĪLTEX - Alternatives to animal experimentation: Vol. The serum-free media interactive online databaseĪLTEX - Alternatives to animal experimentation: Vol. 1 (2018)Īlternatives to the use of fetal bovine serum: Human platelet lysates as a serum substitute in cell culture mediaĪLTEX - Alternatives to animal experimentation: Vol. Article Detailsįetal bovine serum (FBS): Past – present – futureĪLTEX - Alternatives to animal experimentation: Vol. Thus, in order to decrease the annual need for bovine fetuses in terms of the 3Rs through any reduction in the use or partial replacement of serum, as well as in terms of an improvement of cell and tissue culture methodology, serum-free cell culture represents a modern, valuable and scientifically well accepted alternative to the use of FBS in cell and tissue culture.

Besides modern cell biological advances in cell and tissue culture and efforts towards a standardisation of cell culture protocols in Good Cell Culture Practice, in addition, considerable ethical concerns were raised recently about the harvest and collection of fetal bovine serum. Since serum in general is an ill-defined component in cell culture media, a number of chemically defined serum-free media formulations have been developed in the last two decades. The most widely used animal serum supplement is fetal bovine serum, FBS. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. Serum is commonly used as a supplement to cell culture media.

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